Počet záznamů: 1
The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons
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SYSNO ASEP 0354390 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons Tvůrce(i) Chiu, W.-L. (US)
Wagner, Susan (MBU-M) RID, ORCID
Herrmannová, Anna (MBU-M) RID, ORCID
Burela, L. (US)
Zhang, F. (US)
Saini, A. K. (US)
Valášek, Leoš (MBU-M) RID
Hinnebusch, A. G. (US)Zdroj.dok. Molecular and Cellular Biology. - : American Society for Microbiology - ISSN 0270-7306
Roč. 30, č. 18 (2010), s. 4415-4434Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova 40S RIBOSOMAL-SUBUNIT ; SACCHAROMYCES-CEREVISIAE ; IN-VIVO Vědní obor RIV EE - Mikrobiologie, virologie CEP GAP305/10/0335 GA ČR - Grantová agentura ČR CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281287900005 DOI 10.1128/MCB.00280-10 Anotace The C-terminal domain (CTD) of the a/Tif32 subunit of budding yeast eukaryotic translation initiation factor 3(eIF3) interacts with eIF3 subunits j/Hcr1 and b/Prt1 and can bind helices 16 to 18 of 18S rRNA, suggesting proximity to the mRNA entry channel of the 40S subunit. We have identified substitutions in the conserved Lys-Glu-Arg-Arg (KERR) motif and in residues of the nearby box6 element of the a/Tif32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes indicating defects in scanning and start codon recognition. The normally dispensable CTD of j/Hcr1 is required for its binding to a/Tif32 and to mitigate the growth defects of these a/Tif32 mutants, indicating physical and functional interactions between these two domains Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1