Počet záznamů: 1
The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning
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SYSNO ASEP 0354389 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning Tvůrce(i) Cuchalová, Lucie (MBU-M)
Kouba, Tomáš (MBU-M)
Herrmannová, Anna (MBU-M) RID, ORCID
Dányi, István (MBU-M)
Chiu, W.-L. (US)
Valášek, Leoš (MBU-M) RIDZdroj.dok. Molecular and Cellular Biology. - : American Society for Microbiology - ISSN 0270-7306
Roč. 30, č. 19 (2010), s. 4671-4686Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova OPEN READING FRAMES ; START CODON SELECTION ; MESSENGER-RNA Vědní obor RIV EE - Mikrobiologie, virologie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281721400010 DOI 10.1128/MCB.00430-10 Anotace Recent reports have begun unraveling the details of various roles of individual eukaryotic translation initiation factor 3 (eIF3) subunits in translation initiation. Here we describe functional characterization of two essential Saccharomyces cerevisiae eIF3 subunits, g/Tif35 and i/Tif34, previously suggested to be dispensable for formation of the 48S preinitiation complexes (PICs) in vitro. A triple-Ala substitution of conserved residues in the RRM of g/Tif35 (g/tif35-KLF) or a single-point mutation in the WD40 repeat 6 of i/Tif34 (i/tif34-Q258R) produces severe growth defects and decreases the rate of translation initiation in vivo without affecting the integrity of eIF3 and formation of the 43S PICs in vivo. Both mutations also diminish induction of GCN4 expression, which occurs upon starvation via reinitiation Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1