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Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry
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SYSNO ASEP 0354289 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry Tvůrce(i) Vidová, V. (CZ)
Pól, Jaroslav (MBU-M)
Volný, Michael (MBU-M) ORCID
Novák, Petr (MBU-M) RID, ORCID
Wiedmer, S. K. (FI)
Holopainen, J. (FI)Zdroj.dok. Journal of Lipid Research. - : Elsevier - ISSN 0022-2275
Roč. 51, č. 8 (2010), s. 2295-2302Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova ocular lens ; sphingolipid ; matrix-assisted laser desorption/ionization Vědní obor RIV EE - Mikrobiologie, virologie CEP LC07017 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000279896800025 DOI 10.1194/jlr.M005488 Anotace The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyklotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 um thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 um raster. We obtained spatial and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
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