Počet záznamů: 1

Crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein FrpD from Neisseria meningitidis

SYSNO ASEP	0354275
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein FrpD from Neisseria meningitidis
Tvůrce(i)	Sviridova, E. (CZ) Bumba, Ladislav (MBU-M)_{RID, ORCID} Řezáčová, Pavlína (UMG-J)_RID Procházková, Kateřina (UOCHB-X) Kavan, Daniel (MBU-M)_{RID, ORCID} Bezouška, Karel (MBU-M) Kutý, Michal (UEK-B) Šebo, Peter (MBU-M)_{RID, ORCID} Kutá-Smatanová, Ivana (UEK-B)_RID
Zdroj.dok.	Acta Crystallographica Section F-Structural Biology and Crystallization Communications. - : Wiley - ISSN 1744-3091 Roč. 66, - (2010), s. 1119-1123
Poč.str.	5 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Fe-regulated protein D ; iron-regulated proteins ; outer membrane lipoproteins
Vědní obor RIV	EC - Imunologie
CEP	LC06010 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GP310/06/P150 GA ČR - Grantová agentura ČR
CEZ	AV0Z50200510 - MBU-M (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
	AV0Z60870520 - UEK-B (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000281635600035
DOI	10.1107/S174430911003215X
Anotace	Fe-regulated protein D (FrpD) is a Neisseria meningitidis outer membrane lipoprotein that may be involved in the anchoring of the secreted repeat in toxins (RTX) protein FrpC to the outer bacterial membrane. However, the function and biological roles of the FrpD and FrpC proteins remain unknown. Native and selenomethionine-subsituted variants of recombinant FrpD43-271 protein were crystalized using the sitting-drop vapour-diffusion method. Diffraction data were collected to a resolution of 2.25 Å for native FrpD43-271 protein and to a resolution of 2.00 Å for selenomethionine-subsitute FrpD43-271 (SeMet FrpD43-271 ) protein. The crystals of native FrdP43-271 protein belonged to the hexagonal space group P62 or P64, while the crystals of SeMet FrpD43-271 protein belonged to the primitive orthorhombic space group P212121
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2011

Počet záznamů: 1