Počet záznamů: 1
p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex
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SYSNO ASEP 0354237 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex Tvůrce(i) Marcoux, J. (FR)
Man, Petr (MBU-M) RID, ORCID
Petit-Haertlein, I. (FR)
Vives, C. (FR)
Forest, E. (FR)
Fieschi, F. (FR)Zdroj.dok. Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 285, č. 37 (2010), s. 28980-28990Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova SRC HOMOLOGY-3 DOMAINS ; PHOSPHORYLATION-INDUCED ACTIVATION ; TANDEM SH3 DOMAINS Vědní obor RIV CE - Biochemie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281594000063 DOI 10.1074/jbc.M110.139824 Anotace Paper describes mechanism of molecular activation of p47phox, one part of the NADPH oxidase complex. P47phox is a modular multidomain protein that is known to undergo process of activation via phosphorylation. Due to the modular nature and its size it is impossible to solve the structure of autoinhibited and activated form by NMR or crystallography. Therefore we used hydrogen/deuterium exchange coupled to mass spectrometry to describe the structural differences between the two forms. We show that the protein undergoes opening upon activation and described the key regions involved in the interaction in the auto-inhibited state Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1