Počet záznamů: 1
Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA
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SYSNO ASEP 0353565 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA Tvůrce(i) Majerfeld, I. (US)
Chocholoušová, Jana (UOCHB-X) RID, ORCID
Malaiya, V. (US)
Widmann, J. (US)
McDonald, D. (US)
Reeder, J. (US)
Iyer, M. (US)
Illangasekare, M. (US)
Yarus, M. (US)
Knight, R. (US)Celkový počet autorů 10 Zdroj.dok. RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382
Roč. 16, č. 10 (2010), s. 1915-1924Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova SELEX ; molecular modeling ; aptamer ; evolution ; tryptophan Vědní obor RIV CC - Organická chemie CEP ME09019 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000281846500006 DOI 10.1261/rna.2220210 Anotace Conservation is often used to define essential sequences within RNA sites. However, conservation finds only invariant sequence elements that are necessary for function, rather than finding a set of sequence elements sufficient for function. Here we define a critical test of sufficiency: We embed a minimal, apparently sufficient motif for binding the amino acid tryptophan in a random-sequence background and ask whether we obtain functional molecules. After a negative result, we use a combination of three-dimensional structural modeling, selection, designed mutations, high-throughput sequencing, and bioinformatics to explore functional insufficiency. This reveals an essential unpaired G in a diverse structural context, varied sequence, and flexible distance from the invariant internal loop binding site identified previously. Addition of the new element yields a sufficient binding site by the insertion criterion, binding tryptophan in 22 out of 23 tries. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2011
Počet záznamů: 1