Počet záznamů: 1
Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans
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SYSNO ASEP 0353453 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans Tvůrce(i) Wicht, B. (CH)
Yanagida, T. (JP)
Scholz, Tomáš (BC-A) RID, ORCID
Ito, A. (JP)
Jiménez, J. A. (PE)
Brabec, Jan (BC-A) RID, ORCIDZdroj.dok. Journal of Clinical Microbiology - ISSN 0095-1137
Roč. 48, č. 9 (2010), s. 3111-3116Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova MOLECULAR EVIDENCE ; PACIFIC SALMON ; NIHONKAIENSE ; Diphyllobothrium ; multiplex PCR ; the cytochrome c oxidase subunit 1 ; mitochondrial DNA Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína CEP LC522 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000281480400010 DOI 10.1128/JCM.00445-10 Anotace The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing are too complicated, long, and expensive for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1