Počet záznamů: 1
Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii
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SYSNO ASEP 0352314 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii Tvůrce(i) Margos, G. (GB)
Hojgaard, A. (US)
Lane, R. S. (US)
Cornet, M. (FR)
Fingerle, V. (DE)
Rudenko, Natalia (BC-A) RID, ORCID
Aanensen, D. M. (GB)
Fish, D. (US)
Piesman, J. (US)Zdroj.dok. Ticks and Tick-borne Diseases - ISSN 1877-959X
Roč. 1, č. 4 (2010), s. 151-158Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Borrelia bissettii ; Borrelia kurtenbachii sp. nov. ; Ixodes ; Multilocus sequence analysis ; Molecular ecology Vědní obor RIV EC - Imunologie CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000286366600001 DOI 10.1016/j.ttbdis.2010.09.002 Anotace Using multilocus sequence analysis (MLSA), we investigated the phylogenetic relationship of spirochaete strains from North America previously assigned to the genospecies Borrelia bissettii.We amplified internal fragments of 8 housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) located on the main linear chromosome by polymerase chain reaction. Phylogenetic analysis of concatenated sequences of the 8 loci showed that the B. bissettii clade consisted of 4 closely related clusters which included strains from California and Colorado that were isolated from Ixodes pacificus, I. spinipalpis, or infected reservoir hosts. Several strains isolated from I. scapularis clustered distantly from B. bissettii. Genetic distance analyses confirmed that these strains are more distant to B. bissettii than B. carolinensis, a recently described Borrelia species, which suggests that they constitute a new Borrelia genospecies, Borrelia kurtenbachii. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
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