Počet záznamů: 1
Determination of doxazosin and verapamil in human serum by fast LC-MS/MS: Application to document non-compliance of patients
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SYSNO ASEP 0352169 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Determination of doxazosin and verapamil in human serum by fast LC-MS/MS: Application to document non-compliance of patients Tvůrce(i) Chytil, L. (CZ)
Štrauch, B. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCID
Marešová, V. (CZ)
Widimský jr., J. (CZ)
Holaj, R. (CZ)
Slanař, O. (CZ)Celkový počet autorů 7 Zdroj.dok. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 878, č. 30 (2010), s. 3167-3173Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova doxazosin ; verapamil ; hypertension Vědní obor RIV CC - Organická chemie CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000284672300018 DOI 10.1016/j.jchromb.2010.09.032 Anotace A rapid and sensitive method using liquid chromatography–tandem mass spectrometry for simultaneous determination of doxazosin and verapamil in human serum has been developed. Trimipramine-d3 as an isotopic labelled internal standard was used for quantification. Serum samples were prepared by simple liquid–liquid extraction with mixture of tert butyl methyl ether and ethyl acetate. The analytes and internal standard were separated on C18 column using an isocratic elution. Positive TurboIonSpray mass spectrometry was used with multiple reaction monitoring of the transitions. An extensive pre-study method validation was carried out in accordance with FDA guidelines. This assay was successfully applied to determine the serum concentrations of doxazosin and verapamil in suspect non-compliance patients. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2011
Počet záznamů: 1