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In Vitro Antifungal Effect of Black Cumin Seed Quinones against Dairy Spoilage Yeasts at Different Acidity Levels
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SYSNO ASEP 0351528 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In Vitro Antifungal Effect of Black Cumin Seed Quinones against Dairy Spoilage Yeasts at Different Acidity Levels Tvůrce(i) Halamová, K. (CZ)
Kokoška, L. (CZ)
Flešar, J. (CZ)
Skleničková, O. (CZ)
Svobodová, B. (CZ)
Maršík, Petr (UEB-Q) RID, ORCIDZdroj.dok. Journal of Food Protection - ISSN 0362-028X
Roč. 73, č. 12 (2010), s. 2291-2295Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova yeast strains ; culture conditions ; antimicrobial assay Vědní obor RIV GM - Potravinářství CEP GA525/08/1179 GA ČR - Grantová agentura ČR GD525/08/H060 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000285172500021 Anotace The antiyeast activity of the black cumin seed (Nigella sativa) quinones dithymoquinone, thymohydroquinone (THQ), and thymoquinone (TQ) were evaluated in vitro with a broth microdilution method against six dairy spoilage yeast species. Antifungal effects of the quinones were compared with those of preservatives commonly used in milk products (calcium propionate, natamycin, and potassium sorbate) at two pH levels (4.0 and 5.5). THQ and TQ possessed significant antiyeast activity and affected the growth of all strains tested at both pH levels, with MICs ranging from 8 to 128 μg/ml. With the exception of the antibiotic natamycin, the inhibitory effects of all food preservatives against the yeast strains tested in this study were strongly affected by differences in pH, with MICs of ≥16 and ≥512 μg/ml at pH 4.0 and 5.5, respectively. These findings suggest that HQ and TQ are effective antiyeast agents that could be used in the dairy industry as chemical preservatives of natural origin. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2011
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