Počet záznamů: 1
Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata
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SYSNO ASEP 0351175 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata Tvůrce(i) Kobelková, Alena (BC-A)
Bajgar, Adam (BC-A) RID
Doležel, David (BC-A) RID, ORCIDCelkový počet autorů 3 Zdroj.dok. Journal of Biological Rhythms. - : Sage - ISSN 0748-7304
Roč. 25, č. 6 (2010), s. 399-409Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova timeless promoter ; E-box ; Chymomyza costata Vědní obor RIV EB - Genetika a molekulární biologie CEP GP204/08/P579 GA ČR - Grantová agentura ČR GA204/07/1032 GA ČR - Grantová agentura ČR IAA500960802 GA AV ČR - Akademie věd CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000284302400002 DOI https://doi.org/10.1177/0748730410385283 Anotace The tim promoter from D. melanogaster has been studied in detail both in tissue cultures and in living flies yet has never been investigated in other species. This article presents a detailed functional analysis of the tim promoter from the drosophilid fly, Chymomyza costata, in Drosophila tissue cultures. A comparison of tim promoters from wt and npd-mutants confirmed that the 1855 bp deletion in the latter removes crucial regulatory cis-elements as well as the minimal promoter, being subsequently responsible for the lack of tim mRNA expression. Deletion and substitution mutations of the wt tim promoter showed that the region containing the canonical E-box, TER-box, and 2 incomplete E-box sequences is essential for CLK/CYC-mediated expression, while the PERR element appears to be a repressor in S2 cells. Furthermore, the expression of the circadian genes timeless, period, vrille, and doubletime was quantified in C. costata adults. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1