Počet záznamů: 1

Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata

SYSNO ASEP	0351175
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata
Tvůrce(i)	Kobelková, Alena (BC-A) Bajgar, Adam (BC-A)_RID Doležel, David (BC-A)_{RID, ORCID}
Celkový počet autorů	3
Zdroj.dok.	Journal of Biological Rhythms. - : Sage - ISSN 0748-7304 Roč. 25, č. 6 (2010), s. 399-409
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	timeless promoter ; E-box ; Chymomyza costata
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GP204/08/P579 GA ČR - Grantová agentura ČR
	GA204/07/1032 GA ČR - Grantová agentura ČR
	IAA500960802 GA AV ČR - Akademie věd
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000284302400002
DOI	10.1177/0748730410385283
Anotace	The tim promoter from D. melanogaster has been studied in detail both in tissue cultures and in living flies yet has never been investigated in other species. This article presents a detailed functional analysis of the tim promoter from the drosophilid fly, Chymomyza costata, in Drosophila tissue cultures. A comparison of tim promoters from wt and npd-mutants confirmed that the 1855 bp deletion in the latter removes crucial regulatory cis-elements as well as the minimal promoter, being subsequently responsible for the lack of tim mRNA expression. Deletion and substitution mutations of the wt tim promoter showed that the region containing the canonical E-box, TER-box, and 2 incomplete E-box sequences is essential for CLK/CYC-mediated expression, while the PERR element appears to be a repressor in S2 cells. Furthermore, the expression of the circadian genes timeless, period, vrille, and doubletime was quantified in C. costata adults.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2011

Počet záznamů: 1