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Quality control for quantitative PCR based on amplification compatibility test
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SYSNO ASEP 0351162 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quality control for quantitative PCR based on amplification compatibility test Tvůrce(i) Tichopád, Aleš (BTO-N)
Bar, T. (IL)
Pecen, Ladislav (BTO-N)
Kitchen, R.R. (GB)
Kubista, Mikael (BTO-N) RID
Pfaffl, M.W. (DE)Zdroj.dok. Methods. - : Elsevier - ISSN 1046-2023
Roč. 50, č. 4 (2010), s. 308-312Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Quantitative PCR ; Quality control ; Amplification efficiency Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA500520809 GA AV ČR - Akademie věd IAA500970904 GA AV ČR - Akademie věd CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000276262100015 DOI 10.1016/j.ymeth.2010.01.028 Anotace Quantitative qPCR is method for the accurate quantification of nucleic acids. Yet it may generate erroneous results if the amplification process is obscured by inhibition or generation of aberrant side-product. Herein we present a statistical approach based on multivariate analysis of the amplification response data generated in qPCR. The amplification trajectory in its most dynamic phase is fitted with a suitable model. Two parameters of this model, related to amplification efficiency, are then used for calculation of the Z-score statistics. Each studied sample is compared to a predefined reference set of reactions. A probabilistic decision for each individual Z-score is then used to identify the majority of inhibited reactions in our experiments. We demonstrate improved identification performance using the multivariate approach Finally we stress that the performance of the amplification kompatibility test as a quality control procedure depends on the quality of the reference set Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2011
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