Počet záznamů: 1
Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI
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SYSNO ASEP 0351011 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI Tvůrce(i) Řezanka, Tomáš (MBU-M) ORCID
Prell, Aleš (MBU-M)
Spížek, Jaroslav (MBU-M)
Sigler, Karel (MBU-M) RIDZdroj.dok. Journal of Antibiotics. - : Springer - ISSN 0021-8820
Roč. 63, č. 8 (2010), s. 524-529Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. JP - Japonsko Klíč. slova liquid chromatography/MS-ESI ; pilot-plant cultivation ; precursor-directed biosynthesis Vědní obor RIV EE - Mikrobiologie, virologie CEP 1M06011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281499800018 DOI 10.1038/ja.2010.93 Anotace Precursor-directed biogenetic approach was used to produce a range of nonactin homologues in a 50 l fermentor cultivation of strain Streptomyces griseus 34/249 obtained by UV mutagenesis. The production medium contained sodium propionate, isobutyrate and isovalerate as individual precursors, and 10 g l/1 Diaion HP20 styrene-divinylbenzene resin that maintains suitable precursor concentration by reversibly adsorbing and releasing it. The produced nonactin homologues were separated on two C18 reversed-phase liquid chromatography columns in series and analyzed by MS with ESI source in the positive ion mode. Formation of doubly charged ions was suppressed by an excess of Na ions throughout the process. The production of the homologues increased up to day 5 and then it leveled off. Cultivations with individual precursors yielded a total of 18 nonactin homologues whose spectrum depended on the precursor used Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1