Počet záznamů: 1  

Cell ATP level of Saccharomyces cerevisiae sensitively responds to culture growth and drug-inflicted variations in membrane integrity and PDR pump activity

  1. 1.
    SYSNO ASEP0351008
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevCell ATP level of Saccharomyces cerevisiae sensitively responds to culture growth and drug-inflicted variations in membrane integrity and PDR pump activity
    Tvůrce(i) Krasowska, A. (PL)
    Lukaszewicz, M. (PL)
    Bartosiewicz, D. (PL)
    Sigler, Karel (MBU-M) RID
    Zdroj.dok.Biochemical and Biophysical Research Communications. - : Elsevier - ISSN 0006-291X
    Roč. 395, č. 1 (2010), s. 51-55
    Poč.str.5 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaS. cerevisiae ; ABC transporters ; ATP level
    Vědní obor RIVEE - Mikrobiologie, virologie
    CEP1M0570 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    CEZAV0Z50200510 - MBU-M (2005-2011)
    UT WOS000277298000010
    DOI10.1016/j.bbrc.2010.03.133
    AnotaceCellular ATP level in Saccharomyces cerevisiae was measured during culture growth of strain US50-18C overproducing all major PDR pumps and its isogenic mutants variously deleted in these pumps. It was found to be inversely proportional to the intensity of cell metabolism during different growth phases and to the activity of PDR pumps, which are thus among major ATP consumers in the cells. The ATP level was increased when membrane integrity was affected by 0.5% butanol, and further increased by compound 23.1, a semisynthetic phenol lipid derivative that acts as inhibitor of Pdr5p and Snq2p pumps. The magnitude of increase in cell ATP caused by inhibition of Pdr5p pump by compound 23.1 and the Pdr5p pump inhibitor FK506 used for comparison reflects the activity and hence the energy demand of the pump. The rise in cell ATP caused by different PDR pump inhibitors can be thus used as an indicator of pump activity and the potency of the inhibitor
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2011
Počet záznamů: 1  

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