Počet záznamů: 1

New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses

SYSNO ASEP	0350494
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses
Tvůrce(i)	Špak, Josef (BC-A)_{RID, ORCID} Holý, Antonín (UOCHB-X) Pavingerová, Daniela (BC-A) Votruba, Ivan (UOCHB-X)_RID Špaková, Vlastimila (BC-A) Petrzik, Karel (BC-A)_{RID, ORCID}
Celkový počet autorů	6
Zdroj.dok.	Antiviral Research. - : Elsevier - ISSN 0166-3542 Roč. 88, č. 3 (2010), s. 296-303
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Brassica ; Chemotherapy ; Turnip yellow mosaic virus ; Ribavirin
Vědní obor RIV	CE - Biochemie
CEP	GA522/09/0707 GA ČR - Grantová agentura ČR
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000285660300008
DOI	10.1016/j.antiviral.2010.09.019
Anotace	A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2011

Počet záznamů: 1