Počet záznamů: 1
The plant formin AtFH4 interacts with both actin and microtubules, and contains a newly identified microtubule-binding domain
- 1.
SYSNO ASEP 0350394 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The plant formin AtFH4 interacts with both actin and microtubules, and contains a newly identified microtubule-binding domain Tvůrce(i) Deeks, M.J. (GB)
Fendrych, Matyáš (UEB-Q)
Smertenko, A. (GB)
Bell, K.S. (GB)
Oparka, K. (GB)
Cvrčková, F. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Hussey, P.J. (GB)Zdroj.dok. Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533
Roč. 123, č. 8 (2010), s. 1209-1215Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Actin regulating proteins ; Membrane ; Microtubule Vědní obor RIV EB - Genetika a molekulární biologie CEP LC06004 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP305/10/0433 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000276568200004 DOI 10.1242/jcs.065557 Anotace The dynamic behaviour of the actin cytoskeleton in plants relies on the coordinated action of several classes of actin-binding proteins (ABPs). These ABPs include the plant-specific subfamilies of actin-nucleating formin proteins. The model plant species Arabidopsis thaliana has over 20 formin proteins, all of which contain plant-specific regions in place of the GTPase-binding domain, formin homology (FH)3 domain, and DAD and DID motifs found in many fungal and animal formins. We have identified for the first time a plant-specific region of the membrane-integrated formin AtFH4 that mediates an association with the microtubule cytoskeleton. In vitro analysis shows that this region (named the GOE domain) binds directly to microtubules. Overexpressed AtFH4 accumulates at the endoplasmic reticulum membrane and co-aligns the endoplasmic reticulum with microtubules. The FH1 and FH2 domains of formins are conserved in plants, and we show that these domains of AtFH4 nucleate F-actin. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2011
Počet záznamů: 1