Počet záznamů: 1

Mutual regulation of plant phospholipase D and the actin cytoskeleton

SYSNO ASEP	0350391
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Mutual regulation of plant phospholipase D and the actin cytoskeleton
Tvůrce(i)	Pleskot, Roman (UEB-Q)_{RID, ORCID} Potocký, Martin (UEB-Q)_{RID, ORCID} Pejchar, Přemysl (UEB-Q)_{RID, ORCID} Linek, J. (CZ) Bezvoda, R. (CZ) Martinec, Jan (UEB-Q)_{RID, ORCID} Valentová, O. (CZ) Novotná, Z. (CZ) Žárský, Viktor (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Plant Journal. - : Wiley - ISSN 0960-7412 Roč. 62, č. 3 (2010), s. 494-507
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	phospholipase D ; actin ; signaling
Vědní obor RIV	ED - Fyziologie
CEP	IAA601110916 GA AV ČR - Akademie věd
	LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA522/05/0340 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000276659400013
DOI	10.1111/j.1365-313X.2010.04168.x
Anotace	Membrane lipids and cytoskeleton dynamics are intimately inter-connected in the eukaryotic cell and phospholipase D (PLD) and its product phosphatidic acid (PA) were discovered to be important regulators in the membrane–cytoskeleton interface in eukaryotes. Here we report the mechanistic details of plant PLD–actin interactions. Inhibition of PLD by compromises, and PA rescues, pollen tube actin. We identified NtPLDb1 as a regulatory partner of actin, by both activity and in vitro interaction assays. The activity of tobacco PIP2-dependent PLD (PLDb) is specifically enhanced by F-actin and inhibited by G-actin. Using sequence- and structure-based analysis, together with site-directed mutagenesis, we identified Asn323 and Thr382 of NtPLDb1 as the crucial amino acids in the actin-interacting fold. The effect of antisense-mediated suppression of NtPLDb1 or NtPLDd on pollen tube F-actin dynamics shows that NtPLDb1 is the active partner in PLD–actin interplay.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2011

Počet záznamů: 1