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New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss
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SYSNO ASEP 0350371 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss Tvůrce(i) Dyková, Iva (BC-A) RID
Kostka, Martin (BC-A) RID
Wortberg, F. (DE)
Nardy, E. (DE)
Pecková, Hana (BC-A) RID, ORCIDZdroj.dok. Folia Parasitologica. - : Biologické centrum AV ČR, v. v. i. - ISSN 0015-5683
Roč. 57, č. 3 (2010), s. 157-163Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova nodular gill disease ; aetiological study ; amoebae ; Naegleria sp. ; fish diseases ; aquaculture ; Oncorhynchus mykiss Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína CEP GA524/09/0137 GA ČR - Grantová agentura ČR LC522 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000282543400001 DOI 10.14411/fp.2010.021 Anotace We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus on kiss (Walbaum) in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (I), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperolastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
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