Počet záznamů: 1
Gene expression profiling – Clusters of possibilities
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SYSNO ASEP 0349844 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gene expression profiling – Clusters of possibilities Tvůrce(i) Bergkvist, A. (SE)
Rusňáková, Vendula (BTO-N)
Šindelka, R. (GB)
Garda, J.M.A. (ES)
Sjogreen, B. (SE)
Lindh, D. (SE)
Forootan, A. (SE)
Kubista, Mikael (BTO-N) RIDZdroj.dok. Methods. - : Elsevier - ISSN 1046-2023
Roč. 50, č. 4 (2010), s. 323-335Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova qPCR ; Statistical analysis ; Xenopus laevis Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA500970904 GA AV ČR - Akademie věd IAA500520809 GA AV ČR - Akademie věd CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000276262100017 DOI 10.1016/j.ymeth.2010.01.009 Anotace Advances in qPCR technology allow studies of increasingly large systems .The increasing data sizes allow expression profiling both in the gene and the samples dimension results. We distinguish between exploratory and confirmatory statistical studies. we demonstrate several techniques available for exploratory studies on a system of Xenopus laevis development from egg to tadpole. Techniques include hierarchical clustering, heatmap, principal component analysis and self-organizing maps. We stress that even though exploratory studies are excellent for generating hypotheses, results have not been proven statistically significant until an independent confirmatory study has been performed. However, exploratory and confirmatory studies are intimately connected and we would like to raise that awareness among qPCR practitioners. We suggest that scientific reports should always have a hypothesis focus. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2011
Počet záznamů: 1