Počet záznamů: 1

Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat

SYSNO ASEP	0349181
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat
Tvůrce(i)	Šafář, Jan (UEB-Q)_{RID, ORCID} Šimková, Hana (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Čihalíková, Jarmila (UEB-Q)_RID Suchánková, Pavla (UEB-Q)_RID Bartoš, Jan (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581 Roč. 129, 1-3 (2010), s. 211-223
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	BAC library ; Chromosome ; DNA markers
Vědní obor RIV	GE - Šlechtění rostlin
CEP	GA521/07/1573 GA ČR - Grantová agentura ČR
	LC06004 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000280519500023
DOI	10.1159/000313072
Anotace	The large bread wheat genome (1C similar to 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries. Such libraries simplify the task of physical map construction, positional cloning and the targeted development of genetic markers. Rapid improvements in the efficiency and cost of DNA sequencing provide an opportunity to contemplate sequencing the wheat genome by preparing sequence-ready physical maps for each chromosome or chromosome arm in turn. The quality of the chromosome-specific libraries depends on their chromosome coverage and the mean insert size. First-generation libraries suffered from a relatively low mean insert size.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2011

Počet záznamů: 1