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Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement
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SYSNO ASEP 0349037 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement Tvůrce(i) Humpolíčková, Jana (UFCH-W) RID
Benda, Aleš (UFCH-W) RID, ORCID
Macháň, Radek (UFCH-W)
Enderlein, J. (DE)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Physical Chemistry Chemical Physics. - : Royal Society of Chemistry - ISSN 1463-9076
Roč. 12, č. 39 (2010), s. 12457-12465Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fluorescence microscopy ; dunamic saturation optical microscopy ; fluorescence Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy KJB400400904 GA AV ČR - Akademie věd GEMEM/09/E006 GA AV ČR - Akademie věd CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000282643900013 DOI 10.1039/c0cp00059k Anotace Fluorescence microscopy has become one of the most rapidly developing observation techniques in the field of molecular biology, since its high sensitivity, contrast and labeling specificity together with being non-invasive fulfill the most important requirements of live cell imaging. The biggest limitation of the technique seems to be the spatial resolution which is, based on Abbe's diffraction law, restricted to some hundreds of nanometres. Recently, various approaches have been developed that overcome the limit imposed by the diffraction barrier and these methods currently lead the development in the field of fluorescence microscopy. In this contribution, we present dynamic saturation optical microscopy (DSOM) a new technique that monitors the temporal decay of the excited singlet state due to a dark state formation. By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2011
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