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Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds
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SYSNO ASEP 0348486 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds Tvůrce(i) Semenyuk, E.G. (US)
Schmidt, M.A. (US)
Beachy, R.N. (US)
Moravec, Tomáš (UEB-Q) RID, ORCID
Woodford-Thomas, T. (US)Zdroj.dok. Transgenic Research - ISSN 0962-8819
Roč. 19, č. 6 (2010), s. 987-999Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Inducible expression ; Soybean ; Seed-specific genes Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000286110200005 DOI 10.1007/s11248-010-9377-6 Anotace Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFPKDEL reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2011
Počet záznamů: 1