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The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei
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SYSNO ASEP 0347296 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei Tvůrce(i) Paris, Zdeněk (BC-A) RID
Changmai, Piya (BC-A)
RUBIO, M. A. T. (US)
Zíková, Alena (BC-A) RID, ORCID
Stuart, K. D. (US)
Alfonzo, J. D. (US)
Lukeš, Julius (BC-A) RID, ORCIDZdroj.dok. Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 285, č. 29 (2010), s. 22394-22402Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova IRON-SULFUR PROTEINS ; SACCHAROMYCES-CEREVISIAE ; CYSTEINE DESULFURASE ; THIO-MODIFICATION ; FRATAXIN Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/09/1667 GA ČR - Grantová agentura ČR CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000279702200052 DOI 10.1074/jbc.M109.083774 Anotace Fe/S clusters are part of the active site of many enzymes and are essential for cell viability. In eukaryotes the cysteine desulfurase Nfs (IscS) donates the sulfur during Fe/S cluster assembly and was thought sufficient for this reaction. Moreover, Nfs is indispensable for tRNA thiolation, a modification generally required for tRNA function and protein synthesis. Recently, Isd11 was discovered as an integral part of the Nfs activity at an early step of Fe/S cluster assembly. Here we show, using a combination of genetic, molecular, and biochemical approaches, that Isd11, in line with its strong association with Nfs, is localized in the mitochondrion of T. brucei. In addition to its involvement in Fe/S assembly, Isd11 also partakes in both cytoplasmic and mitochondrial tRNA thiolation, whereas Mtu1, another protein proposed to collaborate with Nfs in tRNA thiolation, is required for this process solely within the mitochondrion. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
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