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RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription
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SYSNO ASEP 0347094 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription Tvůrce(i) Kanagaraj, R. (CH)
Huehn, D. (CH)
Mackellar, A. (US)
Menigatti, M. (CH)
Zheng, L. (CH)
Urban, Václav (UMG-J)
Shevelev, Igor (UMG-J)
Greenleaf, A.L. (US)
Janščák, Pavel (UMG-J) RIDZdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 38, č. 22 (2010), s. 8131-8140Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova RECQ5 DNA helicase ; transcription ; genome stability Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/09/0565 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000285420300031 DOI 10.1093/nar/gkq697 Anotace It is known that transcription can induce DNA recombination, thus compromising genomic stability. RECQ5 DNA helicase promotes genomic stability by regulating homologous recombination. Recent studies have shown that RECQ5 forms a stable complex with RNA polymerase II (RNAPII) in human cells, but the cellular role of this association is not understood. Here, we provide evidence that RECQ5 specifically binds to the Ser2,5-phosphorylated C-terminal repeat domain (CTD) of the largest subunit of RNAPII, RPB1, by means of a Set2–Rpb1-interacting (SRI) motif located at the C-terminus of RECQ5. We also show that RECQ5 associates with RNAPII-transcribed genes in a manner dependent on the SRI motif. Notably, RECQ5 density on transcribed genes correlates with the density of Ser2-CTD phosphorylation, which is associated with the productive elongation phase of transcription. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
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