Počet záznamů: 1
Identification of Carbonic Anhydrase I Immunodominant Epitopes Recognized by Specific Autoantibodies Which Indicate an Improved Prognosis in Patients with Malignancy after Autologous Stem Cell Transplantation
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SYSNO ASEP 0347082 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identification of Carbonic Anhydrase I Immunodominant Epitopes Recognized by Specific Autoantibodies Which Indicate an Improved Prognosis in Patients with Malignancy after Autologous Stem Cell Transplantation Tvůrce(i) Skultety, L. (SK)
Jankovičová, B. (SK)
Svobodová, Z. (CZ)
Mader, Pavel (UMG-J)
Řezáčová, Pavlína (UMG-J) RID
Dubrovčáková, M. (SK)
Lakota, J. (SK)
Bílková, Z. (SK)Zdroj.dok. Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893
Roč. 9, č. 10 (2010), s. 5171-5179Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova epitope mapping ; carbonic anhydrase I ; spontaneous remission Vědní obor RIV EB - Genetika a molekulární biologie CEP 1M0505 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA203/09/0820 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000282257800027 DOI 10.1021/pr1004778 Anotace Spontaneous tumor regression was observed in patients who developed aplastic anaemia type syndrome after high-dose therapy with autologous stem cell transplantation and whose sera contained high titer of anti-carbonic anhydrase (anti-CA) autoantibodies. Many indications suggest that presence of these autoantibodies provides significant survival benefit for the patients. Western blot analysis confirmed immunoreactivity of the patients’ sera with several CA isoforms. Highly abundant and widely distributed CA I was selected for epitope mapping study. The applied analytical approach consisted of specific fragmentation of CA I followed by immunospecific isolation of peptides reacting with polyclonal anti-CA I autoantibodies. Standard epitope mapping schema was improved by incorporating magnetic carriers and biomagnetic separation techniques. Mass spectrometry was used for detection and identification of epitopes and acquired results were verified by bioinformatic tools. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
Počet záznamů: 1