Počet záznamů: 1
Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization
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SYSNO ASEP 0346865 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization Tvůrce(i) Yi, Y.J. (KR)
Manandhar, G. (US)
Sutovky, M. (US)
Zimmerman, S.W. (US)
Jonáková, Věra (BTO-N) RID
Leeuwen van, F.W. (NL)
Oko, R. (CA)
Park, Ch.S. (KR)
Sutovsky, P. (US)Celkový počet autorů 9 Zdroj.dok. Cell and Tissue Research - ISSN 0302-766X
Roč. 341, č. 2 (2010), s. 325-340Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Sperm ; Proteasome ; Fertilization ; Zona pellucida ; Acrosome ; Alzheimer's ; Porcine Vědní obor RIV CE - Biochemie CEP 1M06011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA303/09/1285 GA ČR - Grantová agentura ČR NS10009 GA MZd - Ministerstvo zdravotnictví CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000280593600010 Anotace Proteolysis of ubiquitinated sperm and oocyte proteins by the 26S proteasome is necessary for mammalian fertilization. The study examined the role of PSMD4, a subunit of 19S regulatory complex responsible for proteasome-substrate recognition, in sperm-ZP penetration during porcine fertilization in vitro (IVF). Immunofluorescence localized PSMD4 in the sperm acrosome. Immunoprecipitation and proteomic analysis revealed that PSMD4 co-precipitated with porcine sperm-associated acrosin inhibitor (AI). Ubiquitinated species of AI were isolated from boar sperm extracts by affinity purification of ubiquitinated proteins. Some proteasomes appeared to be anchored to the sperm head inner acrosomal membrane. In conclusion, the 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization. The recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for porcine/mammalian fertilization in vitro. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2011
Počet záznamů: 1