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Ribonucleoprotein localization in mouse oocytes
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SYSNO ASEP 0346833 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ribonucleoprotein localization in mouse oocytes Tvůrce(i) Flemr, Matyáš (UMG-J)
Svoboda, Petr (UMG-J) RIDZdroj.dok. Methods. - : Elsevier - ISSN 1046-2023
Roč. 53, č. 2 (2011), s. 136-141Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova mouse oocyte ; in situ hybridization ; immunofluorescence Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/09/0085 GA ČR - Grantová agentura ČR GAP305/10/2215 GA ČR - Grantová agentura ČR ME09039 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000287275900004 DOI 10.1016/j.ymeth.2010.08.005 Anotace RNA molecules rarely function alone in cells. For most RNAs, their function requires formation of various ribonucleoprotein (RNP) complexes. For example, mRNP composition can determine mRNA localization, translational repression, level of translation or mRNA stability. RNPs are usually studied by biochemical methods. However, biochemical approaches are unsuitable for some model systems, such as mammalian oocytes and early embryos, due to the small amounts that can be obtained for experimental analysis. In such cases, microscopic techniques are often used to learn about RNPs. Here, we present a review of immunostaining, fluorescence in situ hybridization with subcellular resolution and a combination of both, with emphasis on the mouse oocyte and early embryos models. Application of these techniques to whole-mount fixed oocytes and early embryos can provide information about RNP composition and localization with three-dimensional resolution. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
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