Počet záznamů: 1
Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography
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SYSNO ASEP 0346483 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography Tvůrce(i) Šindelka, R. (GB)
Šídová, Monika (BTO-N) RID
Švec, David (BTO-N) RID
Kubista, Mikael (BTO-N) RIDZdroj.dok. Methods. - : Elsevier - ISSN 1046-2023
Roč. 51, - (2010), s. 87-91Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Xenopus ; Oocyte ; qPCR tomography Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA500970904 GA AV ČR - Akademie věd GA301/09/1752 GA ČR - Grantová agentura ČR CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000277953300013 Anotace qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined by qPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1 mm) and large amount of total RNA ( 5 μg). Fifteen sections along the animal–vegetal axis were cut and prepared for quantification of 31 RNA targets using the high-throughput real-time RT-PCR (qPCR) BioMark™ platform. mRNAs were found to have two localization patterns, animal/central or vegetal. Because of the high resolution in sectioning, it was possible to distinguish two subgroups of the vegetal gene patterns: germ plasm determinant pattern and profile of other vegetal genes Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2011
Počet záznamů: 1