Počet záznamů: 1

Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells

SYSNO ASEP	0346305
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells
Tvůrce(i)	Mertlíková-Kaiserová, Helena (UOCHB-X)_{RID, ORCID} Votruba, Ivan (UOCHB-X)_RID Matoušová, Marika (UOCHB-X)_ORCID Holý, Antonín (UOCHB-X) Hájek, Miroslav (UOCHB-X)_{RID, ORCID}
Celkový počet autorů	5
Zdroj.dok.	Anticancer Research. - : International Institute of Anticancer Research - ISSN 0250-7005 Roč. 30, č. 7 (2010), s. 2791-2798
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	GR - Řecko
Klíč. slova	acyclic nucleoside phosphonate ; programmed cell death ; CCRF-CEM cells ; cell cycle
Vědní obor RIV	CC - Organická chemie
CEP	1M0508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000280796400043
Anotace	9-[2-(phosphonomethoxy)ethyl] guanine (PMEG) is a guanine acyclic nucleotide analog whose prodrugs are being investigated for chemotherapy of lymphomas. The objective of this study was to determine the requirements for caspase and CD95/Fas activation in PMEG-induced apoptosis and the influence of PMEG on cell cycle regulatory proteins in CCRF-CEM cells. As for cell cycle regulation, cyclin E1 was found to be up-regulated following PMEG treatment. Although an increase of caspase 3, 8 and 9 proteolytic activity was observed, neither pretreatment of the cells with cell-permeable caspase inhibitors nor blocking the death receptor with anti-Fas antibody did prevent apoptosis induced by PMEG. It was concluded that PMEG-induced apoptosis is caspase- and CD95/Fas-independent.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2011

Počet záznamů: 1