Počet záznamů: 1

PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans

SYSNO ASEP	0345859
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans
Tvůrce(i)	Mrázek, Jakub (UZFG-Y)_{RID, ORCID} Koppová, Ingrid (UZFG-Y)_RID Kopečný, Jan (UZFG-Y)_{RID, ORCID} Šimůnek, Jiří (UZFG-Y)_RID Fliegerová, Kateřina (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Folia Microbiologica. - : Springer - ISSN 0015-5632 Roč. 55, č. 4 (2010), s. 352-358
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	CZ - Česká republika
Klíč. slova	Chitosan ; Microbial stability ; Microflora
Vědní obor RIV	FB - Endokrinologie, diabetologie, metabolizmus, výživa
CEP	GA525/08/0803 GA ČR - Grantová agentura ČR
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000281277900012
Anotace	A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2011

Počet záznamů: 1