Počet záznamů: 1

Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

SYSNO ASEP	0345815
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy
Tvůrce(i)	Macháň, Radek (UFCH-W) Hof, Martin (UFCH-W)_{RID, ORCID}
Zdroj.dok.	Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736 Roč. 1798, č. 7 (2010), s. 1377-1391
Poč.str.	15 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	supported lipid bilayer ; giant unilamellar vesicle ; fluorescence recovery after photobleaching
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
CEP	GA203/08/0114 GA ČR - Grantová agentura ČR
	GEMEM/09/E006 GA AV ČR - Akademie věd
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000279365000012
DOI	10.1016/j.bbamem.2010.02.014
Anotace	Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescence correlation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration) making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ...
Pracoviště	Ústav fyzikální chemie J.Heyrovského
Kontakt	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Rok sběru	2011

Počet záznamů: 1