Počet záznamů: 1
A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae
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SYSNO ASEP 0345684 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae Tvůrce(i) Lenz, Ondřej (BC-A) RID, ORCID
Beran, Pavel (BC-A)
Fousek, Jan (BC-A)
Mráz, Ivan (BC-A)Celkový počet autorů 4 Zdroj.dok. Journal of Microbiological Methods. - : Elsevier - ISSN 0167-7012
Roč. 82, č. 1 (2010), s. 90-94Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova microarray ; ITS1 ; mosaicism Vědní obor RIV EE - Mikrobiologie, virologie CEP GP522/07/P338 GA ČR - Grantová agentura ČR CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000279567700014 DOI 10.1016/j.mimet.2010.05.004 Anotace The 16S–23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1