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Filamoeba sp. Isolated from Hot-water Piping System, a Host of Legionella Closely Related to Legionella micdadei
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SYSNO ASEP 0342895 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Filamoeba sp. Isolated from Hot-water Piping System, a Host of Legionella Closely Related to Legionella micdadei Tvůrce(i) Dyková, Iva (BC-A) RID
Hrubá, L. (CZ)
Kostka, Martin (PAU-O)
Pecková, Hana (BC-A) RID, ORCIDZdroj.dok. Acta Protozoologica. - : Jagellonian University - ISSN 0065-1583
Roč. 48, č. 4 (2009), s. 321-327Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova Filamoeba sp. ; intracellular ; Legionella sp. Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína CEP GA524/09/0137 GA ČR - Grantová agentura ČR LC522 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000274737300002 Anotace An amoeba was isolated from 56 ºC hot water, sampled in a piping system that was monitored for the presence of legionellae. The amoeba strain JIH56 was derived from the primary isolate by subculturing at 38ºC. It was assigned to the genus Filamoeba Page, 1967 using light microscopical, ultrastructural and molecular criteria. In the cytoplasm of Filamoeba trophozoites were found Legionella-like bacteria. These were determined using Legionella-specific PCR assay followed by sequencing. Phylogenetic analysis and a similarity search revealed a close relationship of the newly determined legionella sequence with sequences of Legionella micdadei (syn. Tatlockia micdadei), the Pittsburgh pneumonia agent. Phylogenetic analysis of the Filamoeba sequences available to date suggests that the ATCC 50430 strain was misidentified. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
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