Počet záznamů: 1
Real-time monitoring of melittin-induced pore and tubule formation from supported lipid bilayers and its physiological relevance
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SYSNO ASEP 0342768 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Real-time monitoring of melittin-induced pore and tubule formation from supported lipid bilayers and its physiological relevance Tvůrce(i) Macháň, Radek (UFCH-W)
Miszta, Adam (UFCH-W)
Hermens, W. (NL)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Chemistry and Physics of Lipids. - : Elsevier - ISSN 0009-3084
Roč. 163, č. 2 (2010), s. 200-206Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova antimicrobiological peptides ; support phospholipid builayers ; ellipsometry Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GEMEM/09/E006 GA AV ČR - Akademie věd CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000274830200011 DOI 10.1016/j.chemphyslip.2009.11.005 Anotace The temporal evolution of effects of antimicrobial peptide melittin on supported phospholipid bilayers (SPBs) containing negatively charged phospholipids was monitored by ellipsometry and laser scanning microscopy together with measurements of lipid mobility by Z-scan fluorescence correlation spectroscopy. Under all conditions used in our study, we observed reproducibly two effects. The first one is formation of pores in the SPB, which occupy approximately 40% of the bilayer. The formation of pores was accompanied by a decrease in lateral diffusion coefficient of the lipids to approximately 60% of its initial value. The second, simultaneous, effect is the formation of tubules of approximately 30 nm radius and length of the order of 10 mu m. Flushing of the sample with excess of buffer removes most of the tubules, but it does not affect the pores. Further experiments performed under various conditions demonstrated reproducibility of both phenomena. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2011
Počet záznamů: 1