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Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'
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SYSNO ASEP 0342115 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris' Tvůrce(i) Fránová, Jana (BC-A) RID, ORCID
Petrzik, Karel (BC-A) RID, ORCIDCelkový počet autorů 2 Zdroj.dok. Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785
Roč. 158, č. 4 (2010), s. 317-320Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova 16S Ribosomal-RNA ; mycoplasmalike organisms ; gene-sequences Vědní obor RIV EE - Mikrobiologie, virologie CEP 1QS500510558 GA AV ČR - Akademie věd CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000275396600016 DOI 10.1111/j.1439-0434.2009.01615.x Anotace Asparagus officinalis plants with severe fasciation of some spears were observed in southern Bohemia between 1998 and 2007. Nucleic acids extracted from these and asymptomatic plants were assayed with nested polymerase chain reaction (PCR) using the phytoplasma-specific universal ribosomal primers P1/P7 and R16F2n/R2. The restriction profiles obtained from digestion of the PCR products with five endonucleases (AluI, HhaI, KpnI, MseI and RsaI) were identical in all phytoplasmas infecting asparagus in the Czech Republic and indistinguishable from those of phytoplasmas in the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1754 bp of the ribosomal operon indicated that the closest related phytoplasmas were those associated with epilobium phyllody and onion yellows. This is the first report of the natural occurrence of 'Candidatus Phytoplasma asteris' in A. officinalis. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
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