Počet záznamů: 1
Detection of condensin I and II in maturing pig oocytes
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SYSNO ASEP 0341856 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Detection of condensin I and II in maturing pig oocytes Tvůrce(i) Lišková, Lucie (UZFG-Y)
Šušor, Andrej (UZFG-Y) RID, ORCID
Pivoňková, Kateřina (UZFG-Y)
Šašková, Adéla (UZFG-Y)
Karabínová, Pavla (UZFG-Y) RID
Kubelka, Michal (UZFG-Y) RID, ORCIDZdroj.dok. Reproduction Fertility and Development. - : CSIRO Publishing - ISSN 1031-3613
Roč. 22, č. 4 (2010), s. 644-652Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. AU - Austrálie Klíč. slova Chromosome condensation ; Female meiosis ; Phosphorylation Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/06/1297 GA ČR - Grantová agentura ČR GD204/09/H084 GA ČR - Grantová agentura ČR GP524/09/P435 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000276106000007 DOI 10.1071/RD09068 Anotace The multiprotein complexes known as condensins (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Here, we report for the first time the detection of different condensin subunits from both complexes in mammalian oocytes. Using immunoblotting analysis we examined expression levels of condensin subunits during meiotic maturation of porcine oocytes. The expression of the core subunit structural maintenance of chromosomes 2 (SMC2), identical in both condensin complexes, did not change significantly during maturation. Similarly, there was no significant change in the expression of the chromosome associated protein (CAP)-H and CAP-H2 subunits, components of condensin I and II, respectively. Conversely, the expression profiles of CAP-G, CAP-D2 (condensin I) and CAP-D3 (condensin II) were more interesting. At least two isoforms of the CAP-D2 subunit were detected, along with three isoforms of the CAP-D3 and CAP-G subunits. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2011
Počet záznamů: 1