Počet záznamů: 1
Robust Up-Regulation of Nuclear Red Fluorescent-Tagged Fos Marks Neuronal Activation in Green Fluorescent Vasopressin Neurons after Osmotic Stimulation in a Double-Transgenic Rat
- 1.
SYSNO ASEP 0340575 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Robust Up-Regulation of Nuclear Red Fluorescent-Tagged Fos Marks Neuronal Activation in Green Fluorescent Vasopressin Neurons after Osmotic Stimulation in a Double-Transgenic Rat Tvůrce(i) Fujihara, H. (JP)
Ueta, Y. (JP)
Suzuki, H. (JP)
Katoh, A. (JP)
Ohbuchi, T. (JP)
Otsubo, H. (JP)
Dayanithi, Govindan (UEM-P) RID
Murphy, D. (JP)Zdroj.dok. Endocrinology - ISSN 0013-7227
Roč. 150, č. 12 (2009), s. 5633-5638Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova transcription ; protein fusion gene ; hypothalmus Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy CEZ AV0Z50390512 - UEM-P (2005-2011) AV0Z50390703 - UEM-P (2007-2013) UT WOS 000272042300048 DOI 10.1210/en.2009-0796 Anotace The up-regulation in the expression of mRNA or protein encoded by the c-fos gene is widely used as a marker of neuronal activation elicited by various stimuli. To facilitate the detection of activated neurons, we generated transgenic rats expressing a fusion gene consisting of c-fos coding sequences in frame with monomeric red fluorescent protein 1 (mRFP1) under the control of c-fos gene regulatory sequences (c-fos-mRFP1 rats). In these double-transgenic rats, almost all eGFP neurons in the supraoptic nucleus and PVN expressed nuclear mRFP1 fluorescence 90 min after hypertonic saline administration. Furthermore, when combined with transgenes expressing another fluorophore under the control of cell-specific regulatory sequences, activation of specific neuronal cell types in response to physiological cues can be readily detected. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2010
Počet záznamů: 1