Počet záznamů: 1

Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)

SYSNO ASEP	0340099
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)
Tvůrce(i)	Matoušek, Jaroslav (BC-A)_{RID, ORCID} Kocábek, Tomáš (BC-A)_{RID, ORCID} Patzak, J. (CZ) Stehlík, Jan (BC-A) Füssy, Zoltán (BC-A)_{RID, ORCID} Krofta, K. (CZ) Heyerick, A. (BE) Roldán-Ruiz, I. (BE) Maloukh, L. (BE) De Keukeleire, D. (BE)
Celkový počet autorů	10
Zdroj.dok.	Journal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561 Roč. 58, č. 2 (2010), s. 902-912
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	secondary metabolites transcriptional regulation ; cDNA-AFLP analysis ; hop cDNA library screening ; Nicotiana benthamiana ; Petunia hybrida
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA521/08/0740 GA ČR - Grantová agentura ČR
	QH81052 GA MZe - Ministerstvo zemědělství
	ME 940 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000273671900032
DOI	10.1021/jf9043106
Anotace	TFs HlbZIP1A and HlbZIP2 cloned from a cDNA library based on glandular tissue-enriched hop cones are similar to the group A3 bZIP TFs according to characteristic domains. While HlbZIP1A is rather neutral (pl 6.42), HlbZIP2 is strongly basic (pl 8,51). Both bZIP TFs show specific expression in lupulin glands, although low expression was observed also in roots and immature pollen. In a transient expression system, both hop bZIP TFs activated the GUS-coupled promoters of chalcone synthase chs_H1 and O-methyl transferase 1, while the response of the valerophenone synthase promoter to the cloned hop TFs was very low, showing that the cloned bZIP TFs are not strictly G-box-specific. HPLC analysis of secondary metabolites in infiltrated Petunia hybrida showed that both bZIPs interfere with the accumulation and the composition of flavonol glycosides, phenolic acids, and anthocyanins, suggesting the possibility of coregulating flavonoid biosynthetic pathways in hop glandular tissue.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2010

Počet záznamů: 1