Počet záznamů: 1

Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases

SYSNO ASEP	0340098
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases
Tvůrce(i)	Matoušek, Jaroslav (BC-A)_{RID, ORCID} Podzimek, Tomáš (BC-A) Pouckova, P. (CZ) Stehlík, Jan (BC-A) Škvor, J. (CZ) Souček, J. (CZ) Matoušek, Josef (UZFG-Y)_RID
Celkový počet autorů	7
Zdroj.dok.	Oncology Research - ISSN 0965-0407 Roč. 18, č. 4 (2009), s. 163-171
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Anticarcinogenic and antiproliferative nucleases ; Human melanoma ; Tumor xenografts ; Nicotiana benthamina
Vědní obor RIV	FD - Onkologie a hematologie
CEP	GA521/09/1214 GA ČR - Grantová agentura ČR
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000272875000004
Anotace	Antitumor effects and cytotoxicity of recombinant plant nucleases R-TBN1 and R-HBN1 were studied. The nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethyleneglycol. Both nucleases were as effective against human melanoma tumors grown on mouse model as previously studied pine pollen (PN) and mung bean nucleases and their effects were reached at about 10 times lower concentrations compared to bovine seminal RNase (BS-RNase). Because R-HBN1 and R-TBN1 share only 67.4% amino acid identity and only partial immunochemical cross-reactivity, their similar anticancerogenic effects can be explained by their catalytical similarity. Both recombinant nucleases showed lower degree of aspermatogenesis compared to BS-RNase and PN nuclease. Owing to low cytotoxicity on the one hand, and high efficiency on the other, R-HBN1 and R-TBN1 appear to be stable biochemical agents that can be targeted as potential antitumor cytostatics.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2010

Počet záznamů: 1