Počet záznamů: 1  

The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves

  1. 1.
    SYSNO ASEP0337488
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevThe regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves
    Překlad názvuRegulace a mechanismus katalýzy NADP dependentní malátdehydrogenasy (dekarboxylační) z listů tabáku
    Tvůrce(i) Doubnerová, V. (CZ)
    Potůčková, L. (CZ)
    Müller, Karel (UEB-Q) RID, ORCID
    Ryšlavá, H. (CZ)
    Zdroj.dok.Journal of the Serbian Chemical Society - ISSN 0352-5139
    Roč. 14, 8-9 (2009), s. 893-906
    Poč.str.14 s.
    Jazyk dok.eng - angličtina
    Země vyd.CS - CS
    Klíč. slovaNADP-malic enzyme ; macroergic compounds ; Nicotiana tabacum L.
    Vědní obor RIVED - Fyziologie
    CEZAV0Z50380511 - UEB-Q (2005-2011)
    UT WOS000270267200005
    DOI10.2298/JSC0909893D
    AnotaceThe non-photosynthetic NADP-malic enzyme EC 1.1.1.40 (NADP-ME), which catalyzes the oxidative decarboxylation of L-malate and NADP(+) to produce pyruvate and NADPH, respectively, and which could be involved in plant defense responses, was isolated from Nicotiana tabacum L. leaves. The mechanism of the enzyme reaction was studied by the initial rate method and was found to be an ordered sequential one. Regulation possibilities of purified cytosolic NADP-ME by cell metabolites were tested. Intermediates of the citric acid cycle (a-ketoglutarate, succinate, fumarate), metabolites of glycolysis (pyruvate, phosphoenolpyruvate, glucose-6-phosphate), compounds connected with lipogenesis (coenzyme A, acetyl-CoA, palmitoyl-CoA) and some amino acids (glutamate, glutamine, aspartate) did not significantly affect the NADP-ME activity from tobacco leaves. In contrast, macroergic compounds (GTP, ATP and ADP) were strong inhibitors of NADP-ME.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2010
Počet záznamů: 1  

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