Počet záznamů: 1  

AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis

  1. 1.
    SYSNO ASEP0337486
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevAtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis
    Tvůrce(i) Zhang, J. (CN)
    Vaňková, Radomíra (UEB-Q) RID, ORCID
    Malbeck, Jiří (UEB-Q) RID, ORCID
    Dobrev, Petre (UEB-Q) RID, ORCID
    Xu, Y. (CN)
    Chong, K. (CN)
    Neff, M. M. (US)
    Zdroj.dok.PLoS ONE. - : Public Library of Science - ISSN 1932-6203
    Roč. 4, č. 12 (2009), Art_no_-e8236
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaTRANSGENIC TOBACCO PLANTS ; OXIDASE/DEHYDROGENASE GENES ; ISOPENTENYL TRANSFERASE
    Vědní obor RIVED - Fyziologie
    CEPGA206/06/1306 GA ČR - Grantová agentura ČR
    CEZAV0Z50380511 - UEB-Q (2005-2011)
    UT WOS000272829800020
    DOI10.1371/journal.pone.0008236
    AnotaceTwo SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(∆2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2010
Počet záznamů: 1  

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