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Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development
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SYSNO ASEP 0337404 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development Překlad názvu Translace regulovaná mRNA asociovanými faktory v průběhu maturace bovinních oocytů a časného embryonálního vývoje Tvůrce(i) Siemer, C. (DE)
Smiljakovic, T. (DE)
Bhojawni, M. (DE)
Kubelka, Michal (UZFG-Y) RID, ORCID
Tomek, W. (DE)Zdroj.dok. Reproduction in domestic animals. - Malden : Wiley-Blackwell, 2009 / Rodriguez-Martinez Heriberto ; Iaverne Marcel - ISSN 0936-6768 Rozsah stran s. 35-36 Poč.str. 2 s. Akce 42nd Annual Conference of Physiology and Pathology of Reproduction/ 34th Mutual Conference on Veterinary and Human Reproductive Medicine Datum konání 26.02.2009-27.02.2009 Místo konání Leipzig Země DE - Německo Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Oocyte maturation ; Bovine embryo Vědní obor RIV ED - Fyziologie CEP GA524/07/1087 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) Anotace Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. In conclusion, the results indicate that, at the transition from oocyte to embryonic development, translational initiation is regulated by striking differences in the abundance and/or phosphorylation of 5'-end and 3'-end mRNA associated factors, mainly the poly-(A) bindings proteins PABP1 and 3, their repressor PAIP2 and a Maskin-like protein with distinct eIF4E-binding properties which prevents eIF4E/cap binding and eIF4F formation in vitro. Nevertheless, from the M II stage to 16-cell embryos a substantial amount of eIF4E and, to a lesser extent, of eIF4G was precipitated by (7)m-GTP-Separose indicating eIF4F complex formation. Therefore, it is likely that in general the reduction in PABP1 and 3abundance represses overall translation during early embryonic development. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2010
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