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Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development
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SYSNO ASEP 0337303 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development Tvůrce(i) Siemer, C. (DE)
Smiljakovic, T. (DE)
Bhojawni, M. (DE)
Leiding, C. (DE)
Kanitz, W. (DE)
Kubelka, Michal (UZFG-Y) RID, ORCID
Tomek, W. (DE)Zdroj.dok. Molecular Reproduction and Development - ISSN 1040-452X
Roč. 76, č. 12 (2009), s. 1208-1219Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova In Vitro maturation ; Poly(A) binding protein ; Initiation-factor 4E Vědní obor RIV ED - Fyziologie CEP GA524/07/1087 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000271361500012 DOI 10.1002/mrd.21096 Anotace Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2010
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