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Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules
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SYSNO ASEP 0337286 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules Tvůrce(i) Andreeva, Z. (AU)
Ho, A. Y. Y. (AU)
Barthet, M. M. (AU)
Potocký, Martin (UEB-Q) RID, ORCID
Bezvoda, R. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Marc, J. (AU)Zdroj.dok. Functional Plant Biology. - : CSIRO Publishing - ISSN 1445-4408
Roč. 36, č. 7 (2009), s. 600-612Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Allium ; Arabidopsis ; F-actin-microtubule interactions Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA601110916 GA AV ČR - Akademie věd CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000267601900003 DOI 10.1071/FP09024 Anotace Phospholipase D (PLD) is a key enzyme in signal transduction-mediating plant responses to various environmental stresses including drought and salinity. Isotype PLD delta interacts with the microtubule cytoskeleton, although it is unclear if, or how, each of the 12 PLD isotypes in Arabidopsis may be involved mechanistically. We employed RNA interference in epidermal cells of Allium porrum L. (leek) leaves, in which the developmental reorientation of cortical microtubule arrays to a longitudinal direction is highly sensitive to experimental manipulation. Using particle bombardment and transient transformation with synthetic siRNAs targeting AtPLD alpha, beta, gamma, delta, epsilon and zeta, we examined the effect of 'cross-target' silencing orthologous A. porrum genes on microtubule reorientation dynamics during cell elongation. Co-transformation of individual siRNAs together with a GFP-MBD microtubule-reporter gene revealed that siRNAs targeting AtPLD delta promoted, whereas siRNAs targeting AtPLD. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2010
Počet záznamů: 1