Počet záznamů: 1

Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

SYSNO ASEP	0337170
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction
Překlad názvu	Isolace nových konjugátů kyseliny indolyl-3-octové pomocí imunoafinitní extrakce
Tvůrce(i)	Pěnčík, Aleš (UEB-Q)_{ORCID, RID, SAI} Rolčík, Jakub (UEB-Q) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Magnus, V. (HR) Barták, P. (CZ) Buchtík, Roman (UEB-Q) Salopek-Sondi, N. (HR) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Talanta. - : Elsevier - ISSN 0039-9140 Roč. 80, č. 2 (2009), s. 651-655
Poč.str.	5 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Immunoaffinity extraction ; High-performance liquid chromatography (HPLC) ; Tandem mass spectrometry (MS/MS)
Vědní obor RIV	CE - Biochemie
CEP	GD522/08/H003 GA ČR - Grantová agentura ČR
	KAN200380801 GA AV ČR - Akademie věd
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000271701900037
DOI	10.1016/j.talanta.2009.07.043
Anotace	An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol/g fresh weight (IAA) to 0.44 pmol/g fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2010

Počet záznamů: 1