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Focal Adhesion Kinase functions as an Akt downstream target in migration of colorectal cancer cells
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SYSNO ASEP 0335669 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Focal Adhesion Kinase functions as an Akt downstream target in migration of colorectal cancer cells Tvůrce(i) Turečková, Jolana (UMG-J) RID
Vojtěchová, Martina (UMG-J) RID
Krausová, Michaela (UMG-J) RID
Šloncová, Eva (UMG-J)
Kořínek, Vladimír (UMG-J) RIDZdroj.dok. Translational Oncology. - : Elsevier - ISSN 1936-5233
Roč. 2, č. 4 (2009), s. 281-290Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova FAK ; migration ; invasion Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/06/1658 GA ČR - Grantová agentura ČR 2B06077 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) DOI 10.1593/tlo.09160 Anotace Migration plays a crucial role in cancer cell invasion and metastasis. The focus of this study is the collaboration of Akt, FAK and Src kinases in migration and invasion of colorectal cancer cells. We show that all three kinases can be found in one protein complex but the interaction between Akt and Src is indirect and mediated by FAK. Interestingly, induced Akt signaling causes an increased tyrosine phosphorylation of FAK but this increase is attenuated by Src inhibitor SU6656. We also show that active Akt stimulates cell migration, but this phenomenon is blocked by FAK knockdown or by inhibition of Src kinase. In addition, all three kinases appeared crucial for successful invasion of colorectal cancer cells. Alltogether, our data suggest the mechanism how the pathway can affect migration of colorectal adenocarcinoma cells. Since FAK acts downstream of Akt, our results imply FAK kinase as a potential key molecule during progression of tumors with active PI3-K/Akt signaling. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
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