Počet záznamů: 1
Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera
- 1.
SYSNO ASEP 0334950 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera Překlad názvu SELEKTIVNÍ KULTIVACE VYBRANÝCH NOVÝCH DRUHŮ TRYPANOSOMATID IZOLOVANÝCH Z HETEROPTER Tvůrce(i) Yurchenko, V. Y. (US)
Lukeš, Julius (BC-A) RID, ORCID
Jirků, Milan (BC-A) RID
Maslov, D. A. (US)Zdroj.dok. International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 59, č. 43 (2009), s. 893-909Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova gGAPDH ; maximum-likelihood ; spliced leader RNA ; small-subunit rRNA ; trypanosomatids Vědní obor RIV EB - Genetika a molekulární biologie CEP 2B06129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LC07032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000265901700047 DOI 10.1099/ijs.0.001149-0 Anotace Mixed trypanosomatid infections have long been suspected to represent an obstacle for recovering cultures that would faithfully represent original species descriptions. However, without the means to directly compare the parasites in the host and in culture, this would remain just a possibility. Here we have used PCR-based genotyping of spliced leader RNA gene repeats to analyse several novel species of insect trypanosomatids isolated from heteropteran hosts and to compare them with the parasites that had been detected in the gut smears of the same hosts. We have found that most of the axenic cultures contained novel species of Crithidia and Leptomonas. Therefore, we concluded that, in each case, this replacement was caused by differences in cultivation properties between the original predominant blastocrithidia and the less fastidious parasite that was later recovered in culture. The properties of the new organisms were investigated and used to describe five novel species. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2010
Počet záznamů: 1