Počet záznamů: 1
A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae
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SYSNO ASEP 0334906 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae Tvůrce(i) Foldynová-Trantírková, Silvie (BC-A) RID
Matulová, J. (CZ)
Dötsch, V. (DE)
Löhr, F. (DE)
Cirstea, I. (DE)
Alexandov, K. (DE)
Breitling, R. (DE)
Lukeš, Julius (BC-A) RID, ORCID
Trantírek, Lukáš (BC-A)Zdroj.dok. Journal of Biomolecular Structure & Dynamics. - : Taylor & Francis - ISSN 0739-1102
Roč. 26, č. 6 (2009), s. 755-761Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova NMR ; isotope labeling ; protein expression ; Leishmania ; low-level enrichment Vědní obor RIV EB - Genetika a molekulární biologie CEP GP204/08/P585 GA ČR - Grantová agentura ČR 1QS600220554 GA AV ČR - Akademie věd KAN200100801 GA AV ČR - Akademie věd 2B06129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000266300700008 Anotace We report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an economically advantageous alternative to recently established protocol for isotopic labeling using expensive synthetic media. The method is based on cultivation of the L. tarentolae expression strain in a cheap complex medium supplemented with labeled amino acid(s). In this protocol, a labeled amino acid is deliberately diluted in the medium of undefined composition, which leads to alow-level isotope enrichment upon protein over-expression. The economic advantage of the protocol is achieved by avoiding large volumes of expensive synthetic medium. Decreased sensitivity of a NMR experiment due to low-level isotope enrichment is compensated by a five- to seven-fold increase of the yield of the recombinant protein in complex mediumas compared to that in the synthetic medium. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2010
Počet záznamů: 1