Počet záznamů: 1  

A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae

  1. 1.
    SYSNO ASEP0334906
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    NázevA Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae
    Tvůrce(i)Foldynová-Trantírková, Silvie (BC-A) - Matulová, J. (CZ) - Dötsch, V. (DE) - Löhr, F. (DE) - Cirstea, I. (DE) - Alexandov, K. (DE) - Breitling, R. (DE) - Lukeš, Julius (BC-A) - Trantírek, Lukáš (BC-A)
    Rozsah stran755 - 761
    Zdroj.dok.Journal of Biomolecular Structure & Dynamics - ISSN 0739-1102
    Poč.str.7 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaNMR ; isotope labeling ; protein expression ; Leishmania ; low-level enrichment
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPGP204/08/P585 GA ČR - Grantová agentura ČR
    1QS600220554 GA AV ČR - Akademie věd
    KAN200100801 GA AV ČR - Akademie věd
    2B06129 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    CEZAV0Z60220518 - PAU-O, BC-A (2005-2011)
    UTISI000266300700008
    AnotaceWe report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an economically advantageous alternative to recently established protocol for isotopic labeling using expensive synthetic media. The method is based on cultivation of the L. tarentolae expression strain in a cheap complex medium supplemented with labeled amino acid(s). In this protocol, a labeled amino acid is deliberately diluted in the medium of undefined composition, which leads to alow-level isotope enrichment upon protein over-expression. The economic advantage of the protocol is achieved by avoiding large volumes of expensive synthetic medium. Decreased sensitivity of a NMR experiment due to low-level isotope enrichment is compensated by a five- to seven-fold increase of the yield of the recombinant protein in complex mediumas compared to that in the synthetic medium.
    PracovištěBiologické centrum (od r. 2006)
    KontaktVáclava Lavičková, lavickova@hbu.cas.cz, Tel.: 387 775 889
    Rok sběru2010