Počet záznamů: 1
In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer
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SYSNO ASEP 0334099 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer Tvůrce(i) Huranová, Martina (UMG-J) ORCID
Jablonski, J.A. (US)
Benda, Aleš (UFCH-W) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Staněk, David (UMG-J) RID
Caputi, M. (US)Celkový počet autorů 6 Zdroj.dok. RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382
Roč. 15, č. 11 (2009), s. 2063-2071Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova FRET ; FLIM ; RNA-protein interactions Vědní obor RIV EB - Genetika a molekulární biologie CEP KAN200520801 GA AV ČR - Akademie věd LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000270851000014 DOI 10.1261/rna.1678209 Anotace Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA–protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA–protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA–protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA–protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA–protein complexes. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
Počet záznamů: 1